Avaliação in vitro da atividade antiproliferativa de extratos das plantas Jodina rhombifolia HooK et Arn. e Carapa guianensis Aubl sobre células HL-60, Linfoma Daudi e Fibroblastos NIH-3T3

Abstract

Introduction-The incidence of cancer increases annually and is considered one of the main causes of mortality worldwide. Sixty-percent of chemotherapics were obtained from natural sources. Objective - this study aimed to assess the antiproliferative activity of extracts from leaves of Jodina rhombifolia Hook. Et Arn. (methanolic and watery) and seeds of Carapa guianensis Aubl. (methanolic and etheric). Methods - The extract activity was assed by sulforhodamine B colorimetric assay (SRB) on human tumor cell lines of acute myeloid leukemia (HL-60), Daudi Lymphoma and mouse embryonic fibroblasts (NIH-3T3), as control cells. The cell lines were grown using microculture plates and were incubated with five different extract concentrations (2.0, 10.0, 60.0, 100.0 e 600.0 mg/mL) for 72 hours. The anti-proliferative effect was determined by the use of a microplate reader spectrophotometer. With the obtained results a doseresponse curve was generated and the IC50 and ICT values were determined. For statistical analysis the was used with linear regression for different concentrations. The p<0.05 values were considered statistically significant. Results The methanolic extract from Carapa guianensis presented the highest anti-proliferative activity on HL-60 line, with IC50 = 12.6 &#956;g/mL and ICT = 80.5 &#956;g/mL (p<0.05). On Daudi Lymphoma line, the aqueous extract of Jodina rhombifolia presented the best effect: CI50 = 41.09 &#956;g/mL and ICT = 55.90 &#956;g /mL (p<0.05). No statistically significant inhibition was found in control cells (p>0.05). Discussion- The results showed that the extracts obtained from the plants Jodina rhombifolia Hook. et Arn. and Carapa guianensis Aubl. have antiproliferative effect on neoplastic cells of hematopoietic origin. Further studies are necessary to obtain new drugs for clinical use